PNA based artificial nucleases displaying catalysis with turnover in the cleavage of a leukemia related RNA model.
نویسندگان
چکیده
Several peptide nucleic acid based artificial nucleases (PNAzymes) are designed to create a bulge in the target RNA, which is a short model of the leukemia related bcr/abl mRNA. The target RNA is cleaved by the PNAzymes with a half-life of down to 11 h (using a 1 : 1 ratio of PNA-conjugate to target) and only upon base-pairing with the substrate. The PNA based systems are also shown to act in a catalytic fashion with turnover of substrate and are thus the first reported peptide nucleic acid based artificial RNA-cleaving enzymes.
منابع مشابه
Development of Oligonucleotide Based Artificial Ribonucleases 2ʼ-O-MeOBAN’s and PNAzymes
The present thesis is based on three parts. The first and second part describe the development of two different classes of metal-ion dependent artificial ribonucleases, 2'O-methyl-ribonucleic acid based artificial ribonucleases (2'-O-MeOBANs) and peptide nucleic acid based artificial ribonucleases (PNAzymes). These studies may be regarded as a development of traditional antisense methodology. A...
متن کاملTowards artificial ribonucleases: the sequence-specific cleavage of RNA in a duplex.
Lanthanide complexes covalently attached to oligonucleotides have been shown to cleave RNA in a sequence-specific manner. Efficient cleavage, however, is at present limited to single-stranded RNA regions, as RNA in a duplex is considerably more resistant to strand scission. To overcome this limitation, we have designed and synthesised artificial nucleases comprising lanthanide complexes covalen...
متن کاملProgress in Genome Editing Technology and Its Application in Plants
Genome editing technology (GET) is a versatile approach that has progressed rapidly as a mechanism to alter the genotype and phenotype of organisms. However, conventional genome modification using GET cannot satisfy current demand for high-efficiency and site-directed mutagenesis, retrofitting of artificial nucleases has developed into a new avenue within this field. Based on mechanisms to reco...
متن کاملDevelopment of Peptide Nucleic Acid Based Artificial Ribonucleases PNAzymes
Since mRNA plays such a vital role in the transfer of genetic code from DNA to proteins, approaches involving interference with mRNA are of great interest for use in therapeutics. Amongst these, peptide nucleic acid based nuclease systems (PNAzymes) are being developed for in vivo gene silencing in future. The PNAzymes hybridize to the RNA, bringing a catalytic group to the phosphodiester bond ...
متن کاملA highly effective and long-lasting inhibition of miRNAs with PNA-based antisense oligonucleotides.
MiRNAs are non-coding RNAs that play a role in the regulation of major processes. The inhibition of miRNAs using antisense oligonucleotides (ASOs) is a unique and effective technique for the characterization and subsequent therapeutic targeting of miRNA function. Recent advances in ASO chemistry have been used to increase both the resistance to nucleases and the target affinity and specificity ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Organic & biomolecular chemistry
دوره 6 20 شماره
صفحات -
تاریخ انتشار 2008